鲨鱼鳍软骨的胰蛋白酶水解及其“蜂窝式”降解机制

Hydrolysis of shark fin cartilage with trypsin and its “alveolus type” degradation model

研究了胰蛋白酶水解鲨鱼鳍软骨的水解提取液中蛋白质含量、粘多糖含量和硫酸基含量的变化规律 ,并采用扫描电子显微镜分析了鲨鱼鳍软骨在降解过程中的结构变化 ,探讨了胰蛋白酶水解鲨鱼鳍软骨的主要过程和机理 ,首次提出了鲨鱼鳍软骨降解过程的“蜂窝式”降解理论。

The changes in contents of proteins, mucopolysaccharides and sulfate in the supernatant of shark fin cartilage hydrolyzed with trypsin was investigated. Moreover, the microstructures of shark fin cartilage during its gradation were also observed by means of scanning electric microscope. An “alveolus type” model of degradation of shark fin cartilage is firsty suggested on the basis of analysis of its main degradation process. The results showed that the disruption of the compact structures is the basis of its degradation. The degradation process of the cartilage hydrolyzed with trypsin were divided into three periods: the initial, the fast and the slow.