苹果AFLP分析体系的建立

Construction of AFLP Analysis System in Apple

以 12个苹果砧木基因型为试材 ,建立了扩增酶切片段长度多态性 (AFL P)分析体系。对其分析过程中 DNA提取、双酶切、连接、预扩增、标记和选择性扩增的效果进行了检测。用 P32 M6 4引物构建了供试苹果砧木基因型的 AFL P指纹图谱。该指纹图谱拥有扩增条带 10 5条 ,多态性带 6 7条 (占 6 3.8% ) ,条带清晰可辨 ,扩增信号强 ,无背景干扰。讨论了 AFL

AFLP(Amplified Fragment Length Polymorphism)analysis system was established with 12 apple rootstock genotypes( Malus ). The results of several reactions during AFLP analysis have been detected such as DNA extraction,double enzymes restriction,adapter ligation,pre amplification,labeling and selective amplification. The AFLP fingerprintings of 12 apple rootstock genotypes with primer P32M64 have been performed,which had 105 clear and strong amplification bands with bright background. The factors which affect AFLP analysis were discussed.