琼脂扩散沉淀反应检测菌毛抗原的初步研究

Detection of Pili Antigen by Agar Gel Difffusion Precipitin Test

用琼脂扩散沉淀反应检测了大肠杆菌菌毛抗原。结果表明 ,用巴比妥钠 -HCl缓冲液制备的琼脂平板 ,在菌毛孔与抗菌毛血清孔之间出现一条明显的沉淀线 ,而用 80 g/L的NaCl配制的琼脂板在菌毛孔与抗菌毛血清孔之间则无沉淀线。由此提示 ,用巴比妥钠

Avian Escherichia coli pili antigen was detected with agar gel difffusion precipitin test.There was an obvious precipitin band beween pili and anti pili serum hole when using agar plate made by barbital sodium-HCl buffer,but no band in agar plate made by 80 g/L NaCl solution was observed.This showed that agar plate made by barbital sodium-HCl buffer could be used to detect bacterial pili antigen.