皖南烟田三种土传病原物分子检测

Molecular Detection of Three Soil borne Pathogens from Tobacco Field in Anhui Province

为检测皖南烟田土传病害的分布,2011—2012年从皖南烟区烟田共采集土样51份,采用分子生物学方法对烟田土壤进行了烟草黑胫病、根黑腐病和青枯病检测。结果显示,烟田土壤中烟草黑胫病菌、根黑腐病菌和青枯病菌的阳性率分别为19.6%、41.2%和56.9%,3种烟草土传病原菌在皖南烟区多数烟田均有分布,其中青枯病菌检出率最高。本研究证明了应用分子生物学方法检测土传病原菌的可能性,生产上可为烟草种植提供理论依据。

Fifty one of soil samples were collected from southern Anhui Province to detect tobacco soil borne pathogens during 2011-2012. Tobacco soil borne pathogens were mainly composed of Phytophthora parasitica, Thielaviopsis basicola, and Ralstonia solanacearum, which were detected by a molecular biology method. The results demonstrated that the positive rates of P. parasitica, T. basicola and R. solanacearum of 51 samples were 19.6%, 41.2%and 56.9%, respectively. All three diseases occured in Anhui province, but the detection rate of R. solanacearum was the highest among pathogens. This study demonstrates the potential application of molecular biology method in detecting soil borne plant pathogens, which can provide a theoretical basis for tobacco planting in the production.