电针夹脊穴对兔退变腰椎间盘组织中二聚糖表达的影响

Effects of electroacupuncture at jiaji(EX-B2)on the expression of biglycan in rabbit degenerated intervertebral disc

目的:通过研究电针夹脊穴对兔退变的腰椎间盘中二聚糖表达的影响,探讨其作用机制。方法:选用36只清洁级成年新西兰大白兔,建立椎体间压力诱导椎间盘退变的模型,随机分为4组:正常组、假手术组、模型组和治疗组,治疗组给予电针夹脊穴治疗。每组内又分为3个时间点:术前、术后28d、56d;取出椎间盘组织,应用免疫荧光技术检测各组不同时间点二聚糖蛋白的表达。结果:术后28d,模型组、治疗组二聚糖蛋白免疫荧光平均光密度均较假手术组显著下降(均P<0.01);术后56d,治疗组二聚糖蛋白免疫荧光平均光密度较术后28d时明显升高,且较模型组术后56d明显升高(均P<0.05)。结论:椎间盘退变的发生可使二聚糖阳性表达下调,电针夹脊穴可以通过增加二聚糖的阳性表达,纠正基质合成与分解代谢失衡,达到防治椎间盘退变的效果。

Objective：To study the action mechanism of the EA at jiaji points against lumbar disc degeneration.Methods：Thirty-six New Zealand white rabbits were randomly divided into 4 groups：normal group,sham operation group,model group and treatment group.The disc degenernation model was established by using the external dynamic compression device attached to the rabbit lumbar spine for 28 days.Treatment group was given the EA at jiajia point.For each group,the discs were examined radiographically,and double-labelling immunofluorescence was used to detect the expression of biglycan before operation,28th,and 56th day after operation.Result：At 28th day after operation,the expression of biglycan in model group and treatment group was significantly declined as compared with sham operation group（P＜0.01 for all）.At 56th day after operation,the expression of biglycan in treatment group was significantly increased compared to that at the 28th day after operation in model group and treatment group（P＜ 0.05 for all）.Conclusion：EA at jiaji（EX-B2） could protect the degenerated intervertebral discs through up-regulating the expression of biglycan,and correcting the imbalance between extracellular matrix synthesis and degradation.