中国松树枯梢病菌遗传多态性的RAPD分析

THE USE OF RAPD TO DETECT THE GENETIC VARIATION OF SPHAEROPSIS SAPINEA IN CHINA

运用随机扩增多态DNA(RAPD)技术对发生于我国 1 3省区 1 6种松树和其它 2种针叶树上的松枯梢病菌 (Sphaeropsissapinea)的 55个菌株进行基因组DNA多态性分析。用 1 7个随机引物经PCR扩增共得到 2 0 0个RAPD标记 ,其中 98 5%具有多态性。UPGMA聚类分析确定了供试菌株间的亲缘关系 ,将 55个菌株分为 3个类群。各菌株间的差异与其寄主种类无明显关系 ,与其地理来源在某些类群间有一定联系 ,但在大多数菌株间相关趋势不明显。

Genomic DNA from 55 isolates of Sphaeropsis sapinea collected on 16 pines and other 2 conifers from 13 provinces in China was studied using random amplified polymorphic DNA markers(RAPDs). The seventeen random primers produced 200 scorable DNA fragments. One hundred ninety seven fragments were polymorphic. The dendrogram generated by UPGMA divided all isolates into three groups. Cluster analysis showed that differences between isolates were not closely related to the host species and that there were complex relations between the isolate differences and geographic sources.