肝移植前后人血清中蛋白质双向电泳图谱比较

Comparision of spectra of human serum protein on two-dimensional gel electrophoresis before and after liver transplantation

目的研究比较肝移植前后人血清蛋白质双向电泳图谱,探索人肝脏移植后免疫排斥和耐受反应的机制。方法用Aurum Serum Protein Mini Kit对血清预处理,进行双向凝胶电泳(2-DE)分离。Image Master 2D Platinum分析凝胶图谱。结果建立了移植前和移植后24 h、10 d、30 d的人血清双向电泳图谱。与移植前相比,共筛选出明显表达差异的蛋白质斑点15个,其中在移植后蛋白斑点表达上调的14个,表达下调的1个。结论初步建立了肝移植后的人血清蛋白质组学分析方法,差异表达蛋白质点的发现为深入研究肝移植术后免疫耐受和免疫排斥的机制提供了线索。

Objective To compare of the spectra of human serum proteins on two - dimensional gel electrophoresis before and after liver transplantation for studying the mechanism of the immune rejection and immune tolerance after human liver transplantation. Methods Highly abundant proteins in serum were preprocessed using aurum serum protein mini kit and were separated by 2 - DE. The spectra were analyzed by using Image Master 2D Platinum soliware. Results The 2 - D spectra of human serum proteins before and ＇after liver transplantation 24h,10d and 30d were established. Among them 15 protein spots had significant change, 14 protein were up -regulated and one protein was down -regulated. Conclusions Proteomics of human serum after liver transplantation is initially established. The dis- covery of differentially expressed proteins may provide further insight into molecular mechanisms of immune rejection and immune tolerance.