人血管生成素-1真核表达载体的构建与表达

Construction and expression of human angiopoietin-1 eukaryotic vector

目的构建人血管生成素-1的真核表达载体,真核表达人血管生成素-1蛋白并进行纯化。方法通过聚合酶链反应(PCR)将人血管生成素-1基因片段插入B7载体中构建真核表达载体,通过转染中国仓鼠卵巢(CHO)细胞进行真核表达。结果成功构建了B7Ang-1真核表达载体,在CHO细胞中进行了稳定表达,获得了纯化的人血管生成素-1蛋白。结论真核系统表达的人血管生成素-1蛋白具有良好的抗原性,为进一步的生物活性研究和临床应用奠定了基础。

Objective To construct the eukaryotic expression vector of human angiopoietin-1, and express and purify the human angiopoietin-1 protein. Methods The fragment of human angiopoietin-1 gene was inserted into B7 vector by polymerase chain reaction （PCR） and then transfected into Chinese hamster ovary （CHO） cells for eukaryotic expression. Results The B7Ang- 1 eukaryotic vector was constructed and stably expressed in CHO cell. The purified human angiopoietin-1 protein was obtained. Conclusion Human angiopoietin-1 protein is expressed in the eukaryotic system with good antigenicity. This study lays a good foundation for further study on the biological activity and clinical application of human angiopoietin-1.