摘要
目的建立连柏烧伤膏中小檗碱的含量测定方法。方法采用HPLC,色谱柱依利特C18(150mm×4.6mm,5μm),流动相为0.033mol/L磷酸二氢钾溶液-乙腈(60:40),检测波长265nm。结果盐酸小檗碱进样量在0.11~0.33μg之间呈良好的线性关系(r=0.9996),平均回收率为99.51%,RSD为0.99%。结论测定方法简便、准确、重复性好、检测成本低,可用于该产品的质量控制。
Objective To establish a method for the determination of the content of berberine in LianBo Burn cream. Methods HPLC method was used. Hypersil ODS2 C18(150mm×4.6mm,5μm) was used as column. Mobile phase was 0.033 mol/L Potassium dihydrogen phosphate solution-acetonitrile (60: 40). The detection wavelength was 265 nm. Results Berberine hydrochloride had a good linearship between 0.11~0.33μg ( r =0. 999 6 ). The average recovery was 99.51%. RSD was 0.99%. Conclusion The method is simple, accurate, good reproducible and detection of low cost. It can be used for the quality control of Lianbo Burn cream.
出处
《今日药学》
CAS
2013年第11期740-742,共3页
Pharmacy Today