中国西南地区猕猴MHCⅠ类部分等位基因调查

Investigation on Several MHCⅠAlleles of Macaca mulattain Southwestern of China

采用序列特异性引物-聚合酶链反应(PCR-SSP)分型方法对中国西南地区猕猴的MHCⅠ类A*01、A*02、A*08、B*01和NA7等共5个等位基因进行检测。结果显示,在云南猕猴群体中测出了A*01、A*02、A*08、B*01和NA7等位基因,检出率分别为1.27%(2/157)、69.43%(109/157)、11.46%(18/157)、21.02%(33/157)、31.21%(49/157);四川猕猴群检测出了A*02、B*01和NA7等位基因,检出率分别为46.67%(7/15)、13.33%(2/15)、46.67%(7/15);贵州猕猴群检出了A*01、A*02、A*08、B*01和NA7等位基因,其检出率分别为2.5%(2/80)、65%(52/80)、1.25%(1/80)、25%(20/80)、72.5%(58/80);广西猕猴检测出了A*02、B*01和NA7等位基因,检出率分别为60%(6/10)、10%(1/10)、50%(5/10)。中国西南地区猕猴群携带的MHCⅠ类等位基因的频率存在显著性差异(P<0.05)。本研究初步分析了MHCⅠ类等位基因分布情况,为今后开展相关研究奠定了基础。

The A＊01,A＊02,A＊08,B＊01and NA7alleles of MHCⅠof Macaca mulattafrom Southwestern of China were analyzed by PCR-SSP.The frequencies of A＊01,A＊02,A＊08,B＊01and NA7alleles in Yunnan population were 1.27%（2/157）,69.43%（109/157）,11.46%（18/157）,21.02%（33/157）and 31.21%（49/157）,respectively;A＊02,B＊01and NA7alleles in Sichuan were 46.67%（7/15）,13.33%（2/15）and 46.67%（7/15）,respectively;A＊01,A＊02,A＊08,B＊01and NA7alleles in Guizhou population were 2.5%（2/80）,65%（52/80）,1.25%（1/80）,25%（20/80）and 72.5%（58/80）,respectively;A＊02,B＊01and NA7alleles in Guangxi population were 60%（6/10）,10%（1/10）and 50%（5/10）,respectively.There were significant differences in the frequencies of the five alleles of MHCⅠof Macaca mulatta（P〈0.05）.This study analyzed the frequencies of MHCⅠalleles of Macaca mulattafrom Southwestern of China and provided the basis for further studies.