鸡产气荚膜梭菌的分离鉴定及药敏试验

Isolation,Identification and Drug Sensitivity Test of Clostridium perfringens

为确定广东韶关地区两个养殖密度较大鸡场病死鸡的患病病原,根据临床症状、剖检病变疑似坏死性肠炎,每场各分离到1株细菌,通过对细菌分离株进行形态培养、生化鉴定、牛乳汹涌发酵试验、PCR种特异性和毒素型鉴定等试验,鉴定2个分离株均为A型产气荚膜梭菌。对分离株进行药敏试验,测定了临床上常用的抗菌药物添加剂对两株产气荚膜梭菌的最小抑菌浓度(MIC),结果显示恩拉霉素和维吉尼亚霉素对两分离株具有良好的抑菌效果,黄霉素、吉他霉素、硫酸黏杆菌素对个别菌株具有抑菌作用,而那西肽则无明显抑菌作用。研究结果可为鸡坏死性肠炎的临床防控提供参考。

Two strains of bacteria were isolated from resembled necrotic enteritis infectious cases basing on clinical symptom and pathological lesions in two scaled chicken farms in Shaoguan area,Guang dong province.According to the bacterial morphology,biochemical properties,milk fermentation tests,species-specific and toxin PCR tests,the pathogen was identified as A type Clostridium perfringens.We determined Minimum Inhibitory Concentration（MIC）of antibiotic additives used commonly for two strains.The results showed that enramycin and virginiamycin had a better antibacterial effect than flavomycin,kitasamycin and colistin sulfate,and nosiheptide didn′t have an obvious inhibitory effect.This results may provide aguiding significance for the prevention and control of necrotic enteritis in chickens.