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凝血酶敏感蛋白1对体外培养大鼠肺动脉平滑肌细胞的影响

Effect of thrombospondin-1 on proliferation of pulmonary artery smooth muscle cells
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摘要 目的了解凝血酶敏感蛋白1(TSP-1)对体外培养的大鼠肺动脉平滑肌细胞(PASMCs)的影响。方法体外培养大鼠PASMCs,加入不同浓度(10-12、10-11和10-10 mol/L)TSP-1处理12、24、48h,采用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)法检测细胞增殖,流式细胞术(FCM)检测细胞周期。结果 TSP-1刺激后,MMT法实验结果显示TSP-1显著促进大鼠PASMCs增殖,此作用呈浓度和时间依赖性。FCM分析显示TSP-1使S期细胞比例增加。TSP-1处理12h后,S期细胞比例增加,24h时轻微下降,48h时达高峰。结论 TSP-1呈浓度和时间依赖性地促进大鼠PASMCs增殖。 Objective To investigate the effect of thrombospondin 1（TSP-1）on the cell proliferation of cultured rat pulmonary artery smooth cell（PASMCs）in vitro.Methods Rat PASMCs were cultured in vitro,and treated with different concentrations（10^-12、10^-11、10^-10 mol/L）of TSP-1for 12,24,48h.The cell proliferation was quantified by MTT assay.The cell cycle of PASMCs was measured by flow cytometric（FCM）analysis.Results MTT assay showed that TSP-1promoted the proliferation of PASMCs significantly,and the effect was concentration-dependent and time-dependent.FCM analysis indicated that TSP-1increased the percentage of S phase.The percentage of S phase of PASMCs were increased after treated with thrombospondin-1for 12h, slight down after 24h,while reached a maximal level at 48h.Conclusion The TSP-1promotes rat PASMCs proliferation in a concentration-dependent and time-dependent manner.
作者 杨艳娟 杨桂兰 郑西卫 侯嘉 程德云
机构地区 宁夏医科大学总医院呼吸科 四川大学华西医院呼吸科
出处 《重庆医学》 CAS CSCD 北大核心 2013年第35期4294-4296,共3页 Chongqing medicine
基金 国家自然科学基金资助项目(30960142)
关键词 凝血酶敏感蛋白1 肺动脉平滑肌细胞 细胞增殖 thrombospondin 1 pulmonary artery smooth muscle cell cell proliferation
分类号 R543.2 [医药卫生—心血管疾病]
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参考文献14

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共引文献5

重庆医学
2013年 第35期

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