高效液相色谱法测定肝安胶囊中水飞蓟宾的含量

Determination of silybin contents in Gan'an capsule by HPLC

目的建立高效液相色谱法测定肝安胶囊中水飞蓟宾的含量。方法使用Agilent Zorbax SB—C18柱（4．6mm×250mm，5μm），流动相：1％冰醋酸-甲醇=50：50，流速：1．0ml／min，柱温：30℃，检测波长：287nm，测定10批肝安胶囊中水飞蓟宾的含量。结果水飞蓟宾在10．28～123．36μg／ml（r=1）浓度范围内线性关系良好，回收率为97．00％（RSD=0．68％）。10批肝安胶囊中水飞蓟宾含量为11．11～16．65mg／粒。结论该方法简便、准确、重复性好，可以用于肝安胶囊的质量控制。

Objective To establish a HPLC method for determination of silybin contents in Gan＇an capsule. Methods Angilent Zorbax SB C18（4.6 mm ×250 mm,5 μm） column was used with 1% acetic acid ： methanol =50 ： 50 as the mobile phase. The flow rate was 1.0 ml/min,and the temperature was at 30℃. The detection wavelength was 287 nm . Determination of silybin contents in Gan＇an capsule of 10 batches of preparations. Results The silybin calibration curve was at the range of 10.28 - 123.36 μg/ml（r = 1 ） with average recovery of 97.00% （ RSD = 0.68% ）. 10 batches of preparations silybin contents in Gan＇an capsule was 11.11 - 16.65 mg/granule. Conclusion The method was simple accurate and reproducible which could be used for the quality control of Gan＇an capsule.