超声辅助磷酸提取油橄榄叶中羟基酪醇工艺研究

Study on Ultrasound-assisted Phosphoric Acid Extraction Technology of Hydroxytyrosol from Olive Leaves

建立HPLC测定羟基酪醇的方法，通过单因素实验和正交实验优化，以羟基酪醇的含量为考察指标，确定超声辅助磷酸提取羟基酪醇的最佳工艺，研究超声辅助磷酸提取油橄榄叶中羟基酪醇的工艺．实验结果显示：羟基酪醇在3．75—120μg／mL范围内线性关系良好；超声辅助磷酸提取羟基酪醇的最佳奈件为，磷酸浓度为o,2mol／L，液料比为40：l（mL：g），超声时间为60rain，此条件下羟基酪醇的含量为5．13mg/g．该方法工艺简单，处理量大，可为油橄榄叶中羟基酪醇的提取提供依据．

Optimized by single factor test and orthogonal test, taking the content of hydroxytyrosol as evalu- ation index, the experiment was established to determine hydroxytyrosol by HPLC. The experimental results showed that hydroxytyrosol had a good linear relationship with peak area in the range 3.75 ~ 120 btg/mL. When the optimized ultrasonic extraction conditions of hydmxytyrosol were 2 mol/L phosphoric acid, 40：1 liquid-solid ratio （mL： g） and extraction for 60 rain at normal temperature, the hydroxytymsol content was 5.13 mg/g. We conclude that the method is simple, and has large capacity, and can provide the basis for extraction of hydroxytyrosol from olive leaves.