基于检测铜离子的香豆素水合肼希夫碱荧光探针的研究

Study on a coumarin hydrazine schiff base fluorescent probe for copper ion detection

设计合成了一种基于新型香豆素水合肼希夫碱荧光探针,结构经1H NMR表征.利用荧光光谱研究了该探针分子对铜离子的识别作用.实验表明,该探针对铜离子具有较好的选择性识别能力,加入铜离子后,该探针的荧光发射强度显著减弱,并且荧光发射强度随着Cu2+浓度的增加而减弱.通过荧光离子滴定实验对其选择性和抗干扰能力进行了系统研究,发现加入其他常见金属离子(如Al3+、Ca2+、Co2+、Cr3+、Fe3+、K+、Mg2+、Na+、Ni2+、Zn2+等),荧光强度没有发生变化,并且上述各种离子分别与铜离子共存时,对其没有干扰.结果表明,香豆素水合肼希夫碱荧光探针对铜离子具有较高选择性识别.

A novel coumarin derivative was synthesized facilely by the condensation reaction of coumarin and hydrazine hydrate, and its structure was characterized by ^1H NMR. The recognition properties of the target compound 3 with Cu^2＋ had been investigated by the fluorescence spectrophotometry. The results showed that the probe 3 exhibited the selective recognition of Cu^2＋. Upon addition of the concentration of Cu^2＋ to the solution of compound 3, decreasing fluorescence was observed. The selective and interference of compound 3 were discussed. The fluorescence intensity of compound 3 was no chang when the compound 3 was joined other metal ion （such as Al^3＋, Ca^2＋, Co^2＋, Cr^3＋, Fe^3＋. K^＋, Mg^2＋. Na^＋. Ni^2＋, Zn^2＋, etc.）. The interference was found,when other metal and Cu^2＋ coexist in solution of compound 3. Experiments showed that probe 3 was selective recognition of Cu^2＋.