B—NHL患者骨髓单个核细胞表面CD40、CD40L的表达及意义

Expressions and significance of CD40, CD40L in bone marrow mononuclear cell surface of patients with B - cell Non - Hodgkin＇s Lympboma

目的探讨B细胞非霍奇金淋巴瘤（BcellNon—Hodgkin’SLymphoma，B—NHL）患者骨髓单个核细胞（BMMC）表面CIMO、CIM-0L的表达情况及变化规律，分析CIM0、CIMOI。表达与淋巴瘤发病、侵袭性及预后的关系及意义。方法采集20例初诊B—NHL伴有骨髓侵犯的患者（实验组）的骨髓标本及12例健康者（对照组）的骨髓标本，分离单个核细胞，采用直接荧光抗体标记，应用流式细胞仪检测细胞表面C1M0、CIMOL的表达情况。相关实验结果进行统计学比较。结果（1）B—NHL中CIM0阳性率（45．78±3．82）％显著高于正常对照组（13．99±2．20）％，P〈0．01；B—NHL中CIMOL阳性率（3．44±0．88）％显著低于正常对照组（10．64±1．03）％，P〈0．01；（2）B—NHL伴有骨髓侵犯的患者CIM0、CIMOL阳性率与病理类型、LDH值、全身症状呈显著相关，P〈0．05；（3）B—NHL伴有骨髓侵犯的患者中CIMO与CIMOL表达呈负相关，r=-0．47，P〈0．05。结论（1）CIM0、CIM-0L阳性率与病理类型、LDH值、全身症状密切相关，其可能与B—NHL的侵袭性及预后有关；（2）B—NHL患者中CIM0表达的增高、CIMOL表达的减少可能是影响其发病的因素之一；（3）B—NHL患者中CD40L表达的减少可能与肿瘤免疫逃逸机制有关。

Objective To explore the expressions and changing regularity of CIMO,CIMOL in surface of bone marrow mononuclear cell（BMMC） of patients with B -cell Non -Hodgkin＇s Lymphoma. To study the rela- tionship between the expressions of CIM0, CIMOL and the pathogenesis ,infestation ,prognosis, clinical implication of patients with Lymphoma. Methods Twenty bone marrow specimens of B - NHL （ experimental group） and twelve healthy bone marrow specimens （ contiol group） were collected. To separate mononuclear cell, we used di- rect fluorescent antibody to mark cells,the expressions of CIMO and CIMOL were determined by flow cytometry. Statistical study was done between two groups. Results （ 1 ） CIM0 positive overexpression rate in B - NHL sam- pies （45.78± 3.82） % was higher than that in normal samples （ 13.99 ± 2.20） % （ P 〈 O. O1 ） ; CIMOL positive overexpression rate in B - NHL samples （ 3.44 ±O. 88 ） % was lower than that in normal samples （ 10.64 ± 1.03 ） % （P 〈 0. O1 ）. （2）There were significant correlations between the positive overexpression rates of CIM0, CIMOL and pathology category ,lactic dehydrogenase（LDH） levels ,the whole body symptoms in B -NHL patients who suffered from bone marrow involvment（ P 〈 0.05 ）. （3） There were negative correlations between CIMO and CIMOL expressions in B -NHL patients who suffered from bone marrow involvment（ r = -0.47, P 〈 0.05 ）. Conclusions （1）There are close correlations between CD40,CD40L and pathology category, LDH levels and the whole body symptoms,which can likely be correlative in judging the B -NHL prognosis and infestation. （2）High CD40 expressions and low CD40L expressions may probably be one of etiologic factors of B - NHL. （ 3 ） Low CD40L expressions may probably be related with the mechanism of tumor escape.