益心康胶囊对去胸腺衰老大鼠的心肌保护作用的实验研究

EXPERIMENTAL STUDY ON THE PROCTIVE EFFECT OF YIXINKANG CAPSULE ON MYOCARDIUM OF THYMECTOMI ZED RAT

利用４周龄去除胸腺后１５月的大鼠，观察了益心康胶囊（由丹参、黄芪、川芎和山楂组成，简称 Ｈ３０３） 对大鼠心肌抗氧化能力的影响。结果表明：大鼠去除胸腺后心肌组织ＳＯＫ活性明显低于同龄假手术组动物， ＭＤＡ明显增高，心肌组织中的总巯基、非蛋白巯基和蛋白巯基均有一定程度的降低。Ｈ３０３（５０，１００，２００ｍｇ／ｋｇ， 口服 ４Ｗ）能降低动物肿瘤发生率及死亡率；提高机体自由基清除酶ＳＯＤ活力，减少脂质过氧化物 ＭＤＡ及脂褐 素的含量；保护心肌组织巯基。结论：去胸腺大鼠心肌组织抗氧化能力明显降低。Ｈ３０３可通过提高去胸腺大 鼠心肌组织抗氧化能力来发挥其保护心肌的作用。

The effects of Yixinkangcapusle (a traditional Chinese prescription, H303) on myocardium was studied in thymectomized rats (Tx). Rats were thymectomized 4 weeks after birth and used in the experiments 15 months after the operation. The anti-oxidative abilities of the myocardium were observed. It was found that myocardiao SOD activity and the contents of sulfhydryl including total sulfhydryl, non-protein sulfhydryl, protein sulfhydry were significantly decreased, and the content of MDA was significantly increased compared with those of sham-operated rates. Chronic oral administration of H303(50,100,200mg/kg) for consecutive 4 weeks significantly decreased spontaneous incidence of tumor in Tx; prevented the decline of SOD activity, decreased the contents of MDA and lipofuscin, and increased the content of sulfhydryl of the myocardial tissue from Tx. These results suggested that the anti-oxidative ability of myocardial tissue was significantly declined in thymectomized rats and chronic treatment with H303 enhanced the anti-oxidative ability to show protective effects on myocardium in thymectomixze rats.