己酮可可碱在肺缺血-再灌注损伤中的作用

Effects of Pentoxifylline on Ischemia Reperfusion Injury in the Rat Lung

目的 探讨己酮可可碱 (PTX)对肺缺血 -再灌注损伤的保护作用。 方法 72只大鼠随机分为 3组 ,每组 2 4只。 组 :未行缺血及再灌注处理 ; 组 :行左肺缺血和再灌注处理 ; 组 :行左肺缺血和再灌注处理 ,并给予己酮可可碱。采用在体肺温缺血 -再灌注损伤的模型 ,于缺血 45分钟、再灌注 1小时、2小时和 4小时进行动脉血气分析、肺组织含水量、支气管肺泡灌洗液白蛋白含量、血浆和左肺组织丙二醛、左肺组织和支气管肺泡灌洗液髓过氧化物酶(MPO)活性测定。 结果 组再灌注 2小时和 4小时动脉血氧分压显著降低 ,各时间点左肺含水量、支气管肺泡灌洗液白蛋白含量、血浆丙二醛、左肺组织、支气管肺泡灌洗液中髓过氧化物酶均显著升高 ,PTX可改善上述指标变化。结论 PTX通过抑制中性粒细胞肺内聚集 ,减轻肺血管内皮细胞损伤程度 。

Objective To study the effect of pentoxifylline protects against ischemia reperfusion injury to lung. Methods The rat ischemia model with occlusion of left pulmonary hilus for 45 minutes was used in this experiment. Seventy two rats were divided into three groups.Group Ⅰ( n =24): No ischemia; group Ⅱ ( n =24): Ischemia reperfusion; group Ⅲ ( n =24):Pentoxifylline intravenous injection at 5 minutes before ischemia and after 45 minutes of normothermic ischemia. The changes of left lung water indices, malondialdehyde, myeloperoxidase and albumin in the injured lung and brochoalveolar lavage fluid were studied at the time of 1 hour, 2 hours, 4 hours reperfusion after 45 minutes of pulmonary ischemia. Results Group Ⅱ partial pressure of oxygen in artery decreased evidently in 2 hours and 4 hours after reperfusion, left lung water indices, the contents of malondialdehyde, myeloperoxidase and albumin increased evidently at different time points after reperfusion ( P < 0 05). Group Ⅲ, the changes were ameliorated compared with group Ⅱ. Conclusion Pentoxifylline prevented ischemia reperfusion lung endothelium injury and improved postischemia reperfusion lung function by decreasing neutrophil lung sequestration.