先天性膈疝动物模型的制作

The Rat Model of Congenital Diaphragmatic Hernia

目的 制作先天性膈疝的动物模型 ,为进一步研究先天性膈疝合并肺发育不良的病理改变及其处理奠定基础。 方法 妊娠 9.5天的 SD大白鼠随机分成实验组和对照组。实验组经胃一次性灌入 2 ,4-二氯苯基 - P-硝基苯醚 ,对照组经胃灌入等量食用油。妊娠 2 1天时剖腹产取出胎鼠 ,放大镜下观察膈疝形成情况 ,并分别测定胎鼠体重及双肺重量 ,光学显微镜下观察肺的组织学发育情况。 结果 有 2 5只胎鼠制成先天性膈疝模型 ,成功率为 5 8.1% ,实验组胎鼠双肺重量降低 ,组织学显示肺发育不良。 结论 用 2 ,4-二氯苯基 - P-硝基苯醚制作胎鼠膈疝模型具有简便、成功率高的优点 。

Objective To induce congenital diaphragmatic hernia in rats. Methods Nine rats were divided into experimental group( n =6) and control group( n =3), 2,4 dichlorophenyl P nitrophenyl was administrated on the 9.5th day of gestation and the cesarean section was performed on the 21st day of gestation in experimental group. In control group, the same dose of oil was given without 2,4 dichlorophenyl P nitrophenyl. The size and the position of diaphragmatic defect of the fetus were evaluated, and the lung weight and body weight of all fetus were then determined. The morphometrical condition of pulmonary development were assessed with magnification. Results The diaphragmatic defect were found in 25 fetus(58.1%). The lung weight of experimental group was lower than that of the control group, and the pulmonary hypoplasia was found in experimental group. Conclusion The model of congenital diaphragmatic hernia induced by 2,4 dichlorophenyl P nitrophenyl offers a good opportunity to study abnormal lung development in congenital diaphragmatic hernia.