转基因抗草甘膦棉花对草甘膦的耐受性研究

Glyphosate Tolerance Research of Genetically Modified Cotton Resistance to Glyphosate

为了明确转基因抗草甘膦棉花10-6001对除草剂草甘膦的耐受性,以转基因抗草甘膦棉花材料10-6001和常规棉花品种冀棉958(CK)为试验材料,在棉苗6片真叶时叶面喷施不同浓度〔0(不喷施除草剂,人工除草,CK)、0.25%和0.50%〕的草甘膦溶液,分别于施药后0(当天下午)、1、7、14和21 d调查棉花的株高、药害级别、受害指数和死苗率,施药后35 d调查棉花的死苗情况;分析2种棉花对不同浓度草甘膦的反应程度,并对其株高差异进行了显著性方差分析。结果表明:转基因抗草甘膦棉花品系10-6001对草甘膦的耐受性很好,喷施0.25%和0.50%草甘膦溶液的棉花在整个试验期间药害均较轻,表现为植株矮化,生长减慢,叶片略变小,且无死苗,株高方差分析结果显示与人工除草(CK)相比差异均不显著;随着时间的延长,药害逐渐缓解。而常规品种冀棉958对草甘膦的耐受性很差,喷施0.25%和0.50%草甘膦溶液的棉花药害症状表现为植株矮化,生长缓慢,叶片变小、枯黄、褐化干枯,并且药害程度随着时间的延长而加重,药害级别和受害指数均高于10-6001,株高方差分析结果显示与人工除草(CK)相比差异均达到了极显著水平,施药后35 d部分棉花出现整株枯死。

In order to define the tolerance of genetically modified cotton 10-6001 resistance to glyphosate, choose transgenic glyphosate resistant cotton material 106-001 and conventional cotton variety Jimian 958 （ CK ） as test materials, spraying on the leaves with different concentrations of 0 （ CK, no spraying herbicide, artificial weeding） , 0.25% and 0.50% glyphosate solution when cotton seedling stage of 6 true leaves, the plant height, level of the adr, suffer index, death rate were investigated respectively after spraying herbicide 0 （afternoon）, 1, 7, 14 and 21 d, the death of the cotton seedling situation was investigated after spraying herbicide 35 d, and the response of 2 cotton varieties to different concentrations of glyphosate was analyzed , the analysis of variance on plant height was made.The results showed that the cotton seedling stage of 6 true leaves spraying glyphosate, the tolerance of genetically modified cotton 10-6001 to glyphosate was better, the adr of cotton sprayed 0.25% and 0.50% of glyphosate solution was lighter during the whole test, characterized by plant dwarf, growth slow, leaf blade slightly smaller and on death after spraying 35 d.Variance analysis showed that there was no significant difference on plant height with artificial weeding.The adr gradually ease with the extension of time.While glyphosate tolerance of conventional cotton Jimian 958 pairs was very poor, phytotoxicity symptoms of cotton sprayed 0.25%and 0.50% of glyphosate solution were plant dwarf, growth slow, leaves small and yellow and the adr was increased with the extension of time.The level of the adr and the indexes were all higher than that of 10-6001, the plant height difference was very significant with artificial weeding, some cotton died gradually after spraying 35 d.