化香树果序挥发油的气相色谱-质谱联用分析及体外抗肿瘤活性研究

GC-MS Analysis and Antitumor Activity in Vitro of Chemical Constituents of Essential Oil from Platycarya Strobilacea

目的:研究化香树果序挥发油的化学成分及其体外抗肿瘤活性。方法:采用水蒸气蒸馏法从化香树果序中提取挥发油,通过气相色谱-质谱联用技术对其挥发油成分进行分析;采用MTT法观察其对人肝癌HepG2细胞、人鼻咽癌CNE-2细胞及人宫颈癌HeLa细胞的增殖抑制率及半数抑制浓度(IC50)。结果:从挥发油中共鉴定出28个化合物,占总离子流图峰面积的94.10%,其中含量较高的组分为(Z,Z,Z)-9,12,15-十八碳三烯酸(44.48%)、十六烷酸(23.30%)和斯巴醇(3.20%)等。化香树果序挥发油对人肝癌HepG2细胞、人鼻咽癌CNE-2细胞及人宫颈癌HeLa细胞均表现出不同程度的抑制活性,IC50值分别为848.554、451.835、301.253μg/mL。结论:化香树果序挥发油具有多种活性成分,对人肝癌HepG2细胞、人鼻咽癌CNE2细胞及人宫颈癌HeLa细胞均显示出了一定的抑制活性。

Objective： To analyze the chemical constituents of the essential oil from Platycarya strobilacea and investigate the antitumor activity in vitro. Methods： The essential oil of Platycarya strobilacea was extracted by steam distillation. The chem-ical components were separated and analyzed by GC-MS. The proliferations and half inhibition concentration （IC50） were ob-served by MTY assay in hepatocellular carcinoma cell HepG2,nasopharyngeal carcinoma cell CNE2 and cervical carcinoma cell HeLa, which treated with different concentrations of The essential oil of Platycarya strobilacea. Results： There were 28 com-pounds identified and accounted for 94.10% of the peak area from the total ion-current chromatogram. The higher content of components were （Z,Z,Z）-9, 12,15-calendic acid （44.48%）, hexadecanoic acid （23.30%） and barbatol （3.20%）, et al. The essen-tial oil showed different antitumor activities against the proliferation of HepG2,CNE2 and HeLa cells in concentration-dependent manner. The IC50 values of The essential oil of Platycarya strobilacea against HepG2, CNE2 and HeLa cells were 848.554, 451.835,301.253 μg/mL respectively. Conclusion： The essential oil of Platycarya strobilacea contains various constituents which showed antitumor activities on HepG2, CNE2 and HeLa ceils in vitro.