槲皮素在氧化锆修饰玻碳电极上的伏安行为及检测

Voltammetric Characteristics of Quercetin at Zirconia Modified Electrode and its Detection

制备了氧化锆修饰的玻碳电极,采用示差脉冲伏安法和循环伏安法探究了槲皮素在该电极上的电化学行为。结果表明,制备的修饰电极在pH=7.00的磷酸盐缓冲溶液（PBS）中对槲皮素的氧化还原具有明显的电催化作用。采用槲皮素的氧化峰电流作为分析信号。在浓度为2.5×10-8~5×10-5 mol/L的范围内,氧化峰电流和浓度成良好的线性关系,线性方程为ip（μA）=0.0825c-9.861 84,检出限为5.35×10-9 mol/L。

A zirconia modified glassy carbon electrode was prepared.The electrochemical behavior of quercetin at this modified electrode was investigated using differential pulse voltammetry（DPV）and cyclic voltammetry（CV）.The results showed that in the phosphate buffered solution（PBS）with pH =7.00,the electrode brought about obvious catalytic effect on the oxidation-reduction reaction of quercetin.The oxidation peak current of quercetin was chosen as the analytical signal.The intensity of the oxidation peak current has a good linearity with the concentration of quercetin in the concentration range of 2.5×10-8~ 5×10-5 mol/L.The regression equation was ip（μA）=0.0825c-9.86184and the detection limit is 5.35 ×10-9 mol/L.