Triton X-100对漆酶处理苯酚的影响

The Removal of Phenol Catalyzed by Laccase under the Effects of Triton X-100

研究了Triton X-100对漆酶氧化去除水溶液中苯酚的作用.苯酚和漆酶浓度分别是50 mg/L和0.05 mg/mL时,提高苯酚去除率的Triton X-100最佳浓度是155 μM.Triton X-100的浓度从31～930μM时漆酶活性随之增长,在930 μM Triton X-100存在时有最大增值17％.苯酚的初始浓度分别为50,100,200,400和600 mg/L时,155 μM Triton X-100作用下6h后,苯酚去除率分别是对照组的1.2,1.6,3.4,4.5和5.7倍.结果表明Triton X-100可以作为水处理或修复过程的添加剂来提高由漆酶催化的苯酚去除率.

The effects of Triton X-100 on the removal of aqueous phenol catalyzed by laccase were studied.155 μM was the optimal concentration of Triton X-100 to improve phenol removal when the concentrations of phenol and laccase were 50 mg/L and 0.05 mg/mL,respectively.Laccase activity was increased with Triton X-100 at concentrations from 31 to 930 μM and the highest increase was about 17％ by 930 μM Triton X-100.The removal efficiencies of phenol with 155 μM Triton X-100 were 1.2,1.6,3.4,4.5 and 5.7 times of those of the control after 6 h when the initial concentrations of phenol was 50,100,200,400and 600 mg/L,respectively.The reaction of phenol removal was also characterized by using UV spectra.The results indicated that Triton X-100 had application potential for the enhancement of phenol removal catalyzed by laccase in water treatment or remediation.