银杏叶提取物抗氧化作用对大鼠脊髓损伤后运动功能恢复的影响

Antioxidation Effect of Ginkgo Biloba Extract on Motor Function after Spinal Cord Injury in Rats

目的观察银杏叶提取物抗氧化作用对大鼠脊髓损伤后运动功能恢复的影响。方法健康成年Sprague-Dawley大鼠36只,采用Allen法(10 g×25 mm)损伤大鼠T9脊髓节段,复制脊髓损伤动物模型,并随机分为银杏叶提取物治疗组(A组)、甲基强的松龙治疗组(B组)和对照组(C组),每组12只。造模成功后30 min,A组腹腔注射银杏叶提取物40 mg/kg,此后每天1次;B组腹腔注射甲基强的松龙30 mg/kg,此后按5.4 mg/kg每6 h注射1次,共4次;C组每天腹腔注射等体积生理盐水。于术后1 d、3 d、7d随机抽取各组动物4只,进行后肢功能BBB评分及血清丙二醛(MDA)和超氧化物歧化酶(SOD)测定。结果术后各时间点A、B两组BBB评分均高于C组(P<0.05)。术后3 d、7 d,A组、B组动物体内SOD活性均高于C组(P<0.05),而MDA含量均低于C组(P<0.05)。结论银杏叶提取物可降低脊髓损伤大鼠体内的氧化应激水平,促进脊髓损伤大鼠运动功能的恢复。

Objective To observe the antioxidative effect of Ginkgo biloba extract （EGb） on motor function after spinal cord injury （SCI） in rats. Methods 36 healthy adult Sprague-Dawley rats were divided randomly into EGb group （group A）, methylprednisolone （MP） group （group B） and control group （group C） with 12 rats in each group. SCI model was made by Allen＇s mode （10 gx25 mm） at the level of the 9th thoracic vertebra （Tg）. Group A was given 40 mg/kg EGb daily by intraperitoneal injection, while group B was given 30 mg/kg MP at the first injection and then 5.4 mg/kg every 6 hours for 4 times. Group C received an equal volume of normal saline daily. On the 1st, 3rd and 7th days after SCI, 4 rats were selected randomly from each group. The motor function were assessed by Basso-Beattie-Bresnahan scale （BBB）. The activity of superoxide dismutase （SOD） and the content of malondialdehyde （MDA） in serum were determined. Results At ev- ery time point after surgery, the BBB scores were significantly higher in groups A and B than in group C （P〈0.05）. Compared to group C, the activity of SOD increased and the content of MDA decreased significantly in groups A and B on the 3rd and 7th days after SCI （P〈0.05）. Conclusion EGb may reduce the level of oxidative stress and promote the recovery of the motor function in rats after SCI.