近红外脉冲刺激神经的初步实验研究

Preliminary Study on the Pulsed Near Infrared Neural Stimulation

近红外光刺激因其非侵入性、高空间分辨率等优势,成为近年来正在研究的一项新的神经刺激技术。目的:探讨808nm近红外脉冲光刺激神经组织的有效性和安全性。方法:本文中搭建了808nm光刺激实验平台,主要包括808nm激光器、恒流源以及基于MC9S12XE的控制电路,根据实验要求对激光器脉冲输出性能进行了测试;经光纤耦合后,用频率2Hz,脉宽100至1000us脉冲光刺激大鼠初级运动皮层,记录并分析皮层神经元响应。结果:在控制电路调制下激光器脉冲近红外光输出参数稳定;随着脉冲光能量的增加,初级运动皮层神经元spike发放率增加,且在0.2894至2.8939J/cm2阈值范围内光刺激未对神经细胞造成可见性热损伤。结论:本文中实现了808nm激光器脉冲光控制,实验结果初步证实了短波近红外脉冲光刺激中枢神经系统的有效性和安全性。

Infrared neural stimulation （INS） has been proposed as a new neural stimulation technology for free-contract, spatial- ly selective of neural tissue. Objective： Preliminary study the effectively and safety of pulsed infrared light stimulation in neural tis- sue. Methods： First, we set 808nm INS experimental platform, including 808nm laser, constant current source and control circuit based on MC9SI 2XE; secondly, the output performance of pulsed infrared laser were tested; then modulated laser light with repetition rate 2Hz, pulse width 100 - 1000us was coupled to an optical fiber to stimulate the primary motor cortex of SD rat, and the neural re- sponse was recorded at the same time. Results： The output of pulsed laser was stable; spike rate increased with the increase of the stimulation energy; 808nm pulsed INS didn＇ t cause any thermal damage with radiant exposure ranged form0.2894 J/cm2 to 2.8939 J/ cm2. Conclusion： In our study, 808 nm laser pulse light control is realized, and the result preliminary confirmed that short-wavelength pulsed near infrared can be used effectively and safcly to activate central neurol.