紫山药低醇发酵饮料中乙醇含量的气相色谱法测定

Determination of alcohol in purple yam low-alcohol fermented beverage by gas chromatography

建立了紫山药低醇发酵饮料中乙醇含量的气相色谱检测方法。HP-INNOWAX（30m×0．25mm×25ixm）毛细管柱为样品分析柱时，甲醇为样品的稀释剂；正丙醇可作为乙醇定量分析的内标物。甲醇、乙醇、正丙醇的出峰时间分别为2．909、3．110、3．846min，乙醇与正丙醇的分离度大于30。乙醇浓度在0．2-6．0mg／mL范围时，内标法测定的乙醇浓度与乙醇的峰面积的相关系数为0．9996，紫山药样品中乙醇的最低检出限为0．05mg／mL。该测定方法的乙醇回收率在94．4％～107．4％之间，重复性实验的相对标准偏差（RSD）为4．23％，可满足紫山药低醇发酵饮料中乙醇含量的测定要求。

A method for gas chromatography determinnation of alcohol content in purple yam low-alcohol femented beverages was reported in this paper.The samples were separated on HP- INNOWAX （30m x 0.25mm x 251jm） capillary column.Methanol was chosen as the diluent and the n-propanol was used as the standard substance. Results showed that the peak time of methanol, ethanol and n- propanol was 2.909, 3.110 and 3.846min, respectively.And the degree of separation for ethanol and n-propanol was more than 30.The linear range was 0.2-6.Omg/mL with a correlation of 0.9996, and the detection limits were O.05mg/mL for alcohol in purple yam. Recoveries varied from 94.4%-107.4%, and the relative standard deviation（RSD） of the repetitive experimental was 4.23%.This method developed could be used to detect alcohol content in purple yam low-aocohol femented beverages.