厌氧出水培养好氧颗粒污泥及其微生物多样性分析

Cultivation of Aerobic Granules with Effluent from ABR and Analysis of Their Microbial Diversity of Microbial Communities

以处理黄连素废水的厌氧折流板反应器（ABR）出水为进水基质，在序批式反应器（SBR）中培养好氧颗粒污泥，反应器运行80d后形成粒径在2-10mm间的成熟稳定的颗粒污泥。采用扫描电镜（SEM）和聚合酶链式反应一变性梯度凝胶电泳（PCR—DGGE）技术对好氧污泥颗粒化进行分析，考察微生物形态、群落演替和多样性，并对优势菌种进行了分析。DGGE图谱表明，好氧污泥颗粒化进程中，微生物种群呈明显演替变化，好氧颗粒污泥菌群多样性较强。克隆测序结果表明，好氧颗粒污泥反应器优势菌群主要为未分类bacteria、CFBgroupbacteria和Bacteroidetes。

Aerobic granular sludge was cultivated with the effluent of anaerobic baffled reactor （ABR） which degraded berberine wastewater. After 80 d operation, stable granules with sizes of 2-10 mm in SBR were obtained. SEM and PCR- DGGE techniques were used to analyze microbial succession of the sludge. The microbial morphology, population, structure succession process, community diversity and dominant bacteria were investigated. The fingerprint profile of DGGE indicted that population structure succession were obviously during aerobic granulation and bacterial diversity in the aerobic granular sludge was high. DNA sequencing results showed that the dominant community was closely related to bacteria, CFB group bacteria and Bacteroidetes.