P.phragmitetus发酵条件优化及其所产絮凝剂的应用研究

Fermentation Conditions Optimization of P. phragmitetus and Joint Treatment of Activated Dyes Wastewater with Microbial Flocculants and Dicyandiamide-formaldehyde Resin

在LB培养基的基础上,对P.phragmitetus的生长特性进行研究,发现P.phragmitetus所产絮凝剂的表达与菌体呈正相关,且倾向于在碱性条件(pH=7.5)下生长和产生絮凝剂。选择察氏培养基为基础发酵培养基,发现P.phragmitetus产絮凝剂最佳碳源为葡萄糖,氮源为蛋白胨,最适碳氮比为4∶1。以该菌所产絮凝剂粗品和脱色剂双氰胺甲醛树脂对模拟的活性染料废水进行联合处理。结果表明,微生物絮凝剂投加量为40μg/mL,脱色剂用量为100μg/mL时,脱色率达到90%。相对于传统化学处理工艺,该联合处理工艺所需脱色剂和絮凝剂用量都更少。

The comparative study of growth characteristics of P. phragmitetus cultured in LB medium has found that the flocculating activity accumulation is parallel with cell concentrations and P. phragmitetus prefers to grow and produce flocculants in alkaline culture of pH 7.5. Based on Czapek medium, it finds that the optimum carbon source, nitrogen source are glucose, peptone, with C：N as 4：1. The crude microbial flocculants and dicyandiamide-formaldehyde resin are used to the joint treatment for the simulating wastewater of activated dyes. Result shows that when the dosages of microbial flocculants and dicyandiamide-formaldehyde resin are 40μg/mL and 100 μg/mL respectively, the decolorization rate is beyond 90%. Compared with traditional treatment with chemical reagents, the joint treating technique needs less decolorant and flocculants in practice.