两种微藻对高浓度酵母发酵废水中细胞耐受能力的比较

Comparison of Cell Tolerance Capacity of Two Microalgae Species to High Concentration Wastewater from Yeast Fermentation

本文研究了蛋白核小球藻（Chlorella pyrenoidosa，简称C. py）和二形栅藻（Scenedesmus dimorphus NIES-119，简称NIES-119）在异养生长中对蔗糖的耐受浓度，进而比较了对高浓度酵母发酵废水的细胞耐受性。结果表明，C. py和NIES-119在蔗糖浓度高达70 g/L的培养基中仍有高比生长速率（0.90 d-1和0.63 d-1），最大生物量浓度可达到3.37 g/L和2.84 g/L。酵母废水添加糖蜜后，COD可高达104~105 mg/L并伴随有总氮、总磷的高浓度，C. py和NIES-119在这类废水培养基中的最高比生长速率为0.43 d-1和0.42 d-1，最大生物量浓度可达到1.95 g/L和1.70 g/L，均极显著高于不加糖蜜的废水培养基中的最高值；同时，C. py对废水培养基中COD、总氮、总磷的最高去除率分别高达34.56%、20.00%和20.63%，NIES-119则分别高达21.46%、31.25%和16.11%。结果说明这两种微藻在耐受高浓度酵母废水、通过生长净化废水中都具有巨大潜力。

In the present work, the heterotrophic growth of Chlorella pyrenoidosa and Scenedesmus dimorphus NIES-119 were analyzed to investigate the tolerant concentrations of sucrose and the cell tolerance capacity to high concentration wastewater from yeast fermentation. The results showed that Chlorella pyrenoidosa and Scenedesmus dimorphus NIES-119 had high specific growth rates （0.90 d-1 and 0.63 d-1, respectively） in medium containing high concentration of sucrose up to 70 g/L, and the maximal biomass concentrations reached to 3.37 g/L and 2.84 g/L, respectively. After adding molasses in the wastewater medium, the chemical oxygen demand （COD） of 104~ 105 mg/L was observed with high concentration of total nitrogen and phosphorus. The maximal specific growth rates and highest biomass concentrations were 0.43 d-1 and 1.95 g/L, respectively, for Chlorella pyrenoidosa, and were 0.42 d-1 and 1.70 g/L, respectively, for Scenedesmus dimorphus NIES-119, which were significantly higher than the maximum in the molasses-free wastewater medium. Furthermore, the highest removal rates of COD, total nitrogen and total phosphorus were 34.56%, 20.63% and 20.00%, respectively, by Chlorella pyrenoidosa and 21.46%, 16.11% and 31.25%, respectively, by Scenedesmus dimorphus NIES-119. These resultsdemonstrated the two kinds of microalgae had feasibility and great potential for wastewater cleaning.