三七叶总皂苷酶解产物抗抑郁活性的评价

Antidepressant Effects of the Enzymatic Products from the Total Saponins in Panax notoginseng Leaves

本文研究了三七叶总皂苷酶解产物EP-3的抗抑郁药效。采用强迫游泳、悬尾、获得性无助、利血平拮抗等行为模型以及甩头实验、奔跑实验、打斗实验进行评价。强迫游泳实验，EP-3可以使强迫游泳小鼠的不动时间缩短43.39~52.00%，具有明显的抗抑郁活性。获得性无助实验，EP-3能显著减少获得性无助小鼠的逃避失败次数，测定的第2 d EP-3给药组逃避失败次数减少了16.56~29.96%，测定的第3 d减少了20.06~35.28%，第4 d为29.63~62.82%，呈现出随给药时间延长，作用增强的特点。EP-3可以明显逆转利血平诱导的小鼠肛温下降和拮抗小鼠眼睑下垂，一定程度上缓解小鼠运动不能症状。此外，EP-3显著增加5-HTP诱导的小鼠甩头次数，减少可乐定诱导的小鼠打斗行为，提高L-Dopa诱导的小鼠奔跑速度。但是EP-3对悬尾实验小鼠的不动时间没有影响（P〉0.05）。EP-3具有良好的抗抑郁作用，其作用机制可能与5-HT、NE和DA等单胺能神经递质或其受体有关。

To evaluate the antidepressant effects of the enzymatic products EP-3 from the total saponins in leaves of Panax notoginseng, a series of animal models and tests, such as the forced swim test （FST）, the tail suspension test （TST）, learned helplessness procedure, reserpine-induced syndrome, head-twitch test, running and aggressive behaviors were used. EP-3 could shorten the immobility time of FST mice for 43.39% to 52.00% that showed an obvious antidepressant-like effect. EP-3 could significantly reduce the times of escape failures in the model of learned helplessness procedure, which was reduced by 16.56% to 29.96% and 29.63% to 62.82% on day 2 and 4, respectively. EP-3 could significantly reverse the reduction in rectal temperature and the degree of palpebral ptosis, and moderately remit the condition of akinesia induced by reserpine. Moreover, EP-3 could significantly increase the times of head twitches induced by 5-HTP, decrease the times of aggression induced by clonidine and improve the running behavior induced by L-Dopa. However, EP-3 had no effect on the immobility time of TST mice （P〉0.05）. In conclusion, EP-3 exerts excellent antidepressant-like effects that maybe have a close relationship with the monoamine neurotransmitters 5-HT, NE, DA or their receptors.