不同波长UV对副溶血性弧菌光修复反应的影响

Effect of Different Wavelength Ultraviolet Radiation on Photoreactivation in Vibrio parahaemolyticus

低压水银管是常见的灭菌工具,其一般采用的是波长为254 nm的UVC作为灭菌光源。而UVA(365 nm波长)配合发光二极管(light-emitting diodes,LED)也有明显的杀菌效果。本研究从灭菌能力,照射后细菌的光修复能力和再生长能力以及能耗等方面入手,比较短波长的UVC和长波长的UVA/LED对副溶血性弧菌的灭菌效果。结果显示,UVA/LED比UVC有更强的杀菌能力,且在受到UVA/LED照射后,副溶血性弧菌的再生长明显慢于单纯UVC处理后的细菌;对副溶血性弧菌的光修复酶基因表达水平进行检测发现,UVA/LED照射后副溶血性弧菌三种光修复酶基因的表达水平都无激活现象,而UVC处理后的细菌三种基因表达水平明显升高。我们认为UVA/LED可能抑制副溶血性弧菌的光修复现象。但在具备以上优势的同时,UVA/LED的能耗大大高于UVC,因此该技术还有待进一步改进。

The low pressure mercury lamp is used as a common tool for disinfection, with wavelength 254 nm as light source. Previous results suggested that when connected with light-emitting diodes （LED）, UVA could kill bacterial effectively. In this research, we found that the disinfection effect of UVA/LED was much stronger than that of UVC; meanwhile, the regeneration of Vibrio parahaemolyticus after radiated by UVA/LED was more slowly than that only exposed by UVC. The results showed that photoreactivation did not happen in Vibrio parahaemolyticus after radiation by UVA/LED, and gene expression of photoreactivation related enzymes was not induced either. However, after UVC radiation, the photoreactivation happened and the gene expression level of photolyase increased. Thus results indicated that UVA/LED might inhibit photoreactivation in vibrio paraheamolyticus. Nevertheless, UVA/LED equipment needs much higher energy than UVC disinfection device, which should be improved.