用AFLP标记鉴定带有簇毛麦抗白粉病基因的小麦易位系

Identification of Haynaldia villosa Translocation Lines with Resistant Gene to Powdery Mildew in Wheat by AFLP Analysis

对 4个小簇麦及小麦亲本和抗源供体簇毛麦进行了AFLP分析 ,确定了 4个小簇麦是均含有一段簇毛麦DNA的易位系。从得到的 3个与该基因可能较紧密连锁的标记和 7个不太紧密连锁的标记中 ,推测 4个易位系中簇毛麦DNA的长短不一样。文中还讨论了AFLP作为一种准确。

To identify the resistance for powdery mildew from Haynaldia villosa in 4 wheat Haynaldia villosa lines,we analysed them and their parents(common wheat,Taiyuan 768 and Haynaldia villosa) by AFLP method.The results suggested that 4 wheat Haynaldia villosa lines were all translocations and their resistant genes come from Haynaldia villosa.From 3 markers probably close linked to the resistant gene and 7 markers it is infered that the lengths of DNAs from Haynaldia villosa are different.AFLP is a reliable and efficient method for the identification of translocation.