多小穗小麦品系10-A及其改良系的谷蛋白分析

Glutenins Analysis of Multispikelet Wheat Line 10-A and Its Recombinants

应用十二烷基硫酸钠聚丙烯酰胺凝胶电泳 (SDS -PAGE)分析了多小穗小麦品系 10 -A以及来源于三交组合 10 -A/ 88- 16 43/川育 12号的 5 6个稳定品系的谷蛋白。结果表明 ,10 -A、88- 16 43和川育 12号的谷蛋白带纹各不相同 ,能彼此区分。在这 5 6个后代品系中 ,高分子量谷蛋白带纹出现了与 88- 16 43、川育 12号一致的类型以及 4种三亲本间的重组类型 ,没有出现 10 -A的谱带类型和突变类型。在这些品系中 ,有 8个品系含优质亚基组合2 ,7+ 8,5 + 10 (占 14 3% ) ,11个品系含优质亚基组合 1,7+ 8,5 + 10 (占 19 6 % )。

The glutenin patterns of multispikelet wheat line 10-A and its recombinants were analyzed by using sodium dodeeyl sulphate polyacrylamide gel electrophoric (SDS-PAGE). From the glutenin patterns, 10-A, 88-1643 and Chuanyu 12 could be identified by each other. In the 56 lines derived from triple cross 10-A/88-1643/Chuanyu 12, Six HMW-glutenin patterns are observed, including the same patterns with 88-1643 and Chuanyu 12 and the patterns among 3 parents. There are eight lines with good glutenin subunits 2 *, 7+8, 5+10(14 3%) and eleven lines with good glutenin subunits 1, 7+8, 5+10(19 6%).