基于代谢组学的厚朴与枳实配伍减毒机制分析

Detoxicating mechanism of Cortex Magnoliae Officinalis and Fructus Aurantii Immaturus combination based on metabonomic studies

目的利用1H NMR为基础的代谢组学研究厚朴及厚朴配伍枳实的肝肾毒性。方法 18只大鼠随机分成3组:空白组、厚朴组和厚朴枳实组。以1H NMR为基础的代谢组学分析空白组、厚朴组和厚朴枳实组大鼠尿液和血清样品代谢轮廓,同时进行肝、肾组织病理学检查。结果厚朴组和空白组样品代谢轮廓具有明显差异,厚朴枳实组的代谢轮廓同空白组样品接近,肝、肾组织病理学检查同时证实了数据分析。结论厚朴与枳实配伍使用能够有效减少厚朴对肝肾所产生的毒性。

Objective Magnolia offwinalis Rehd. et Wils. （ Hou Po） is one of the most popular traditional Chinese medicine. It has been used to treat a wide variety of clinical diseases such as wind-stroke, headache, cold and heat, fright qi, blood impediment and dead muscle. In this study, 1H NMR-based metabonomies approach was used to investigate the toxicity of Houpo and Houpo com- bined with Fructus Aurantii Immaturu~ （ Zhi Shi ）. Methods Male Wistar rats were divided into three groups ： control, group of Houpuo, group of Houpo combined with Zhishi. Based on 1 H NMR spectra of urine and serum from rats, partial least-squares discrimi- nant analysis（PLS-DA） was performed to identify different metabolic profiles. Liver and kidney histopathology examinations were also performed. Results PLS-DA scores plots demonstrated that the metabolic profile treated with Houpo alone was different from that of the control rats. The metabolic profile of the group treated with Houpo combined with Zhishi was similar with that of the control. Statis- tics results were confirmed by the histopathological examination. Conclusion The combination of Houpo and Zhishi would relieve the damage in liver and kidney caused by Houpo.