LCGR在山羊星状神经节的分布及其意义

Distribution of Luteinizing Hormone/Choriogonadotropin Receptor in the Stellate Ganglia of Goat and Its Implications

【目的】检测促黄体激素/绒毛膜促性腺激素受体(luteinizing hormone/choriogonadotropin receptor,LCGR)在山羊星状神经节(stellate ganglion,SG)中是否存在,探讨促黄体激素(luteinizing hormone,LH)和绒毛膜促性腺激素(choriogonadotropin,CG)是否可以影响星状神经节的活动。【方法】取雄性和雌性成年山羊的星状神经节各5对,经免疫组织化学SP法后,观察LCGR在星状神经节的分布特点,Image-Pro Plus 6.0(IPP 6.0)软件图像半定量分析技术,分析星状神经节中的神经元和非神经元的LCGR分布差异。【结果】山羊星状神经节的神经元和非神经元均有不同程度的阳性染色。LCGR强阳性产物分布于神经元的胞质中和神经突起上,但细胞核中无LCGR分布;卫星细胞、血管内皮细胞的胞质中有弱阳性产物,LCGR在神经元和非神经元中的表达量呈极显著性差异(P<0.01)。【结论】LCGR受体广泛分布于山羊星状神经节中,且主要分布于神经元的细胞质中和细胞膜上,星状神经节具备接受LH和CG调节的条件。

[Objective] The present study was carried out to examine the existence of LCGR （luteinizing hormone/ choriogonadotropin receptor, LCGR） in the stellate ganglion （SG） of goat. [Method] Five pairs of stellate ganglions （SG） were taken from mature female and male goats. The distribution characteristics of LCGR were studied after immunohistochemical SP staining. Image-Pro Plus 6.0 （IPP 6.0） was used as the method of semi-quantitative image analysis to evaluate the expression difference of LCGR between neurons and non-neuron cells. [Result] Neurons and non-neuron cells in SG were immunolabelled in different degrees. In the neurons, uniformly strong immunostaining was found in the whole cytoplasm and neurites, whereas no receptors were detected in the nucleus. In the non-neuron cells including satellite cells and vascular endothelial cells, the cytoplasm was weakly immunolabelled. A significant difference in the intensity of immunolabelling could be found between neurons and non-neuron cells. [Conclusion] LCGR was widely distributed in SG of goat and mainly in the cytoplasm and on the membrane of neurons, suggesting that LH and CG may exert potential effects on SG.