T型分叉中精氨酸-甘氨酸-天冬氨酸三肽涂层材料聚乙二醇-聚乳酸-聚谷氨酸共聚物对人脐静脉内皮细胞黏附稳定性的研究

Effect of RGD peptide on adhesive stability of human umbilical vein endothelial cell on polyethylene terephthalate surface in the T-shape bifurcation

目的研究T型分叉血管对RGD肽对内皮细胞(endothelial cell,EC)在涂层材料聚乙二醇-聚乳酸-聚谷氨酸共聚物(PEG-PLA-PGL)表面黏附稳定性的影响。为该材料作为冠状动脉内支架涂层材料的临床应用提供实验依据。方法实验材料(PEG-PLA-PGL)分为两组:RGD组(表面共价接枝人工合成的RGD三肽)、对照组(共聚物组),然后在两组材料表面种植体外培养的人脐静脉EC,并在固定常流条件下观察比较在T型管不同位置、不同流速下RGD肽对材料表面细胞黏附稳定性的影响。结果作用4 h后,在共聚物组及RGD组T型管分叉前(A)、分叉后(B)、分叉(C)三处位置上EC细胞残余率在最大流速(0.8 m/s)下分别为(14.98±1.45)%vs.(17.38±2.52)%、(10.06±2.12)%vs.(14.25±2.03)%、(11.08±2.18)%vs.(13.27±1.89)%。发现三组存在明显统计学差异(P<0.05)。共聚物表面结合RGD后,细胞残余率明显增加。但是无论在对照组还是在RGD组,T型分叉前A位置EC细胞残余率都要比分叉及分叉处的细胞残余率高(P<0.05)。结论 RGD可以提高EC在共聚物材料表面的黏附稳定性,但是T型分叉对EC的黏附稳定性有一定的影响。

Objective Study the effect of RGD Peptide on Adhesive Stability of Human Umbilical Vein Endothelial Cell on PEG-PLA-PGL copolymer surface in the T-shape Bifurcation to provide experimental evidence for for RGD as the coating material of coronary stent in clinical application. Methods PEG-PLA-PGL copolymer were divided into two groups：RGD group （covalently grafting synthetic tripeptide RGD） and control group （copolymer）, and the 2 groups of implants surface cultured human umbilical vein endothelial cells, and in steady flow conditions observe and compare of different locations, different flow rates RGD peptide fixed in the T-tube on the surface of cell adhesion stability. Results After 4 h action, the control groups and the RGD groups [in the T-tube at the position A （front fork）, B （after bifurcation）, C （bifurcation）] under maximum flow rate （0.8 m/s）, the EC residual rate respectively for the 14.98%±1.45% vs. 17.38%±2.52%, 10.06%±2.12% vs. 14.25%±2.03%, 11.08%±2.18%vs. 13.27%±1.89%. Found that three groups exist significant difference （P〈0.05）. In the RGD groups, the EC residual rate was more compared with the control groups. However, both in the control groups and the RGD groups, T-type front fork position A EC residual ratio better than the position B and C （P〈0.05）. Conclusion RGD can improve endothelial cell adhesion stability in the copolymer material surface, but the T-shaped bifurcation do has a certain impact on the stability adhesion of the EC.