脊髓慢性压迫损伤动物模型实验研究

Empirical study of chronic compressed spinal cord injury model in animal

目的建立一种理想、实用的慢性脊髓压迫模型,为进一步研究慢性压迫性脊髓损伤的病理生理机制奠定基础。方法利用自行设计一种脊髓压迫装置制作大鼠慢性压迫模型,通过术后大鼠后肢运动功能BBB评分、X线片、脊髓HE染色评价该模型的可靠性。结果 X线片示1周脊髓受压程度约21%,3周后脊髓受压程度58%。脊髓功能受损存在一定的隐匿性,3周压迫组BBB评分与对照组无明显差异(P=0.193),6、9周压迫组BBB评分持续降低,3个压迫组间存在显著差异(P<0.05)。HE染色示受压脊髓灰质神经元丢失,白质脱髓鞘改变,病变随受压时间的延长逐渐加重,3组间脊髓前角神经元密度存在显著性差异(P<0.05)。结论此装置较好地模拟了慢性脊髓压迫症的临床特征,具有取材方便,方法简单、科学、重复性强的优点。

Objective To establish an ideal and practical model of chronic spinal compression, so as to provide a basis for further studies on pathophysiologic mechanism of chronic compressive spinal cord injury. Methods The model of chronic spinal compression was made with a self-designed device, postoperative BBB score of hind limb motor function of rat, X-ray picture, and HE stain of spinal cord were used to evaluate reliability of this model. Results X-ray picture showed that the degree of spinal compression was 21%at 1 week and 58%at 3 weeks. The damage of spinal cord function was delitescenced, BBB score had no difference between 3 weeks＇ compression and control group, the BBB score of 6, 9 weeks＇ compressive groups decreased persistently.The difference were significant between 3 groups of compression （P〈0.05）. Neuron loss in grey matter and demyelination in white matter was found at compressive spinal cord stained with HE and pathological change aggravated with compression of time extension.The density of neuron in cornu anterius medullae spinalis have significant difference between 3 groups of compression （P〈0.05）. Conclusion This self-designed device stimulate the clinical feature of chronic compressive spinal cord injury which have some virtues of convenient, simple, scientific, repetitive.