人工龋树脂牙本质粘接界面拟生态矿化的定量评估

Quantitative evaluation of biomimetic remineralization of artificial dentinal caries

目的探讨人工龋拟生态再矿化的定量评估方法和效果。方法通过脱矿/再矿化液的pH循环法建立人工龋模型,使用全酸蚀粘接剂Single Bond Plus(SB组)、One-Step(OS组)和自酸蚀粘接剂Adper Prompt L-Pop(LP组)进行树脂粘接,在含聚丙烯酸(PAA)和聚乙烯膦酸(PVPA)的模拟体液/硅酸盐水门汀系统中诱导矿化,采用显微计算机X线断层摄影术(micro CT)定量评估再矿化效果。结果矿化诱导4个月后SB组、OS组和LP组样本病损深度均有降低,由矿化前的300μm分别减少至47、53和87μm,矿化率分别为73.76%、81.39%和74.54%。结论 micro CT是定量评估人工龋树脂牙本质粘接界面矿化程度的一种有效方法。含PAA和PVPA的模拟体液/硅酸盐水门汀系统可诱导树脂渗透脱矿牙本质再矿化(P<0.05),且不同的粘接剂对人工龋拟生态再矿化效率无显著性影响。

Objective To investigate the quantitative evaluation of biomimetic remineralization of artificial dentinal caries. Methods Artificial dentin caries lesion was established by pH cycling with demineralization and remineralization fluid. The caries-like dentin was bonded with （1） Single Bond Plus （SB）, an etch-and-rinse adhesive using moist bonding; （2） One-Step （OS）, an etch-and-rinse adhesive using moist bonding; （3） Adper Prompt L-Pop （LP）, a self-etching adhesive. All specimens were remineralized by Portland cement/simulated body fluid remineralization media by incorporating polyacrylic acid and Polyvinyl phosphonic acid as biomimetic analogs of matrix proteins. Lesion depth and integrated mineral loss were quantitatively monitored and evaluated with microcomputed tomography. Results After 4 months＇ remineralization, the depth of lesions of group SB, OS and LP were reduced to 47, 53 and 87 μm, with the remineralization rate of 73.76%, 81.39% and 74.54%. Remineralization was achieved in three groups. Conclusions Quantitative evaluation of biomimetic remineralization of artificial dentinal caries with micro CT is feasible; The degree of remineralization of the three groups enhanced significantly with no significant difference.