姜黄素对非酒精性脂肪性肝病大鼠肝组织HO-1表达的影响

Effects of curcumin on expression of liver heme oxygenase-1 in non-alcoholic fatty liver disease rats

目的观察姜黄素对高脂饮食诱导的非酒精性脂肪性肝病(NAFLD)大鼠肝组织血红蛋白氧合酶-1(HO-1)的影响。方法 SD大鼠36只随机分为正常组、模型组和姜黄素组(每组12只)。正常组全程饲以普通饲料,另两组均给予高脂饮食。于6周末每组各处死2只大鼠。验证NAFLD造模成功后,给予姜黄素组50 mg·kg-1·d-1姜黄素灌胃,正常组、模型组给予相应体积的0.5%羧甲基纤维素钠(CMC)灌胃。6周后,留取肝组织,免疫组织化学法测定肝组织HO-1的蛋白含量,荧光定量PCR法检测肝组织HO-1的mRNA表达。结果与正常组、模型组相比,姜黄素组大鼠肝组织内HO-1蛋白和mRNA表达均显著增高(P值均<0.01)。结论姜黄素可诱导NAFLD大鼠肝组织内HO-1的表达,可能是其防治NAFLD的机制之一。

Objective To observe the effects of curcumin on expression of liver heme oxygenase-1 in high-fat diet induced non-alcoholic fatty liver disease rats. Methods Thirty-six SD rats were randomly divided into the normal group, model group and curcumin group with twelve in each group. The rats in the normal group were fed with a standard diet while those in other two groups received a high-fat diet. Two rats from each group were executed at the end of the sixth week to detect the pathological changes. Then, the rats in the curcumin group were gavaged with 50 mg/kg of curcumin daily while those in the normal group and model group received an equal volume of 0.5% CMC as control. At the end of the twelfth week, all rats were executed. The protein and mRNA expressions of liver heine oxygenase-1 （HO-1） were determined by immunohistochemistry and RT-PCR. Results Compared with the normal group and model group, the protein and mRNA expressions of liver HO-1 were significantly higher in the curcumin group （P〈0.01）. Conclusion Curcumin can induct the expression of liver HO-1, which may be one of its mechanisms for prevention and treatment of nonalcoholic steatohepatitis.