摘要
对红酵母RY - 981细胞壁破碎及其类胡萝卜素浸提的方法进行了较详细的筛选和条件优化研究。结果表明 :酸热法是红酵母破壁较理想的方法 ,其最优条件组合为盐酸浓度 2mol/l、盐酸用量 1 2ml/ g菌体、盐酸浸泡时间 40min和沸水浴处理 4min;丙酮是从红酵母细胞碎片浸提类胡萝卜素较优越的有机溶剂 ,其参考用量为 2 0ml/g菌体。在此破壁和浸提条件下 ,红酵母RY - 981类胡萝卜素提取率可达 30 7 9μg/ g菌体。
The methods of breaking cell walls and extracting carotenoids were studied.The experimental results showed that the method of combining hydrochloric acid(HCl)with heating(by boilng water)was suitable for the cell rupturing from rhodotorula RY-981,and its optimal conditions were:HCl concentration 2 mol/l,HCl addition 12 mL/g(dry cells),soaking time 40 min and heating time 4 min.Using acetone of 20 mL/g(dry cells)was the most extracting method.Under the above optimal conditions,the yield of carotenoids could reched to 307.9 μg/g(dry cells).
出处
《食品与机械》
CSCD
2000年第6期30-31,共2页
Food and Machinery
基金
河南省杰出青年科学基金项目! (80 1 2 1 )
河南省自然科学基金项目! (9940 1 2 50 0 )
