超高效液相色谱-串联质谱法测定辣椒制品中酸性橙Ⅱ

Determination of acid orangeⅡin pepper products by ultra performance liquid chromatography-tandem mass spectrometry

建立了辣椒制品中酸性橙Ⅱ的超高效液相色谱-串联质谱分析方法。样品用乙腈提取，WAX弱阴离子固相萃取柱净化浓缩，经超高效液相色谱分离，三重四极杆质谱电喷雾电离（ESI），负离子多反应监测模式检测。结果表明，在1—200ng／mL质量浓度范围内，线性相关系数大于0．998；加标回收率为83．2％～113．2％，相对标准偏差为0．7％-7．2％。检出限分别为1．0μg／kg（辣椒油及辣椒酱）和2．0μg／kg（辣椒粉）。方法适用于辣椒制品中酸性橙Ⅱ的测定。

A method for the determination of acid orange II in hot pepper products by ultra performance liquid chromatography-tandem mass spectrometry was established. The samples were extracted with acetonitrile and pu- rifled by WAX SPEs. The separation was target compound was detected by MS/MS carried out by ultra performance liquid c system with electrospray ionization （ESI hromatography （UPLC）. The ） under multiple reaction mo-nitoring （MRM） mode. The calibration curve showed good linearity in the range of 1 - 200 ng/mL with correla- tion coefficient better than 0. 99. The average recoveries ranged from 83.2% to 113.2% at the spiking levels of 2 ~ 200 μg/kg with RSDs of 0. 7% ~ 7.2%. The limit of quantitation （LOQ） was 1.0 μg/kg for chili oil and chili sauce and 2. 0 μg/kg for chili power, respectively. The method is fast-speed, high sensitivity and could be applied to quantitative determination of acid orange II in pepper products.