高稳定性安培型葡萄糖生物传感器的研究

Development of an amperometric glucose biosensor with high stability

采用有机改性溶胶凝胶-聚乙烯醇(Ormosil-PVA)包埋葡萄糖氧化酶,制备普鲁士蓝修饰玻碳电极,并基于此制备了安培型葡萄糖生物传感器。以含有FeCl3,K3Fe(CN)6和EDTA的溶液为沉积液,通过循环伏安电沉积法制备了高稳定性的普鲁士蓝修饰电极。考察了扫描电位范围、外加电位、pH和温度等因素对传感器的影响。葡萄糖浓度在20μmol/L^2 mmol/L范围内与响应电流呈线性关系,相关系数R=0.9965,以3倍空白值的标准偏差计算此传感器的检出限为8.1μmol/L。其中,在低浓度(20~100μmol/L)范围内,电流与浓度也呈良好的线性关系(R=0.9938)。

An amperometric glucose biosensor was developed based on the immobilization of glucose oxidase in organically modified silicate （Ormosil）-polyvinyl acetate （PVA） matrix onto a Prussian Blue （PB）-modified glassy carbon electrode. A highly stable PB modified electrode was prepared by the electrochemical deposition of FeCl3, K3 [ Fe （CN） s ] and ethylenediamine tetraacetic acid （EDTA） using cyclic vohammetry （CV）. The effects of the scanning potential range, applied potential, pH and temperature were investigated. The detection limit of the glucose biosensor was 8.1 μmol/L （ S/N = 3 ） with a linear range from 20 μmol/L to 2 mmol/L （ R = 0. 9965）. Within the range of low concentration from 20 μmol/L to 100 mmol/L, the glucose biosensor still presented a good linear relationship （R = 0. 9938 ）. The biosensor had a fast response and good selectivity. Ad- ditionally, excellent reproducibility and stability of the biosensor were observed.