摘要
一氧化氮(NO)是一种在植物次生代谢中起着重要调控作用的信号分子.我们以硝普钠(SNP)作为NO供体处理新疆紫草愈伤组织.实验表明,SNP处理的新疆紫草红色愈伤组织中AePGT,AeGPPS,AeC4H与Ae4CL的表达量比未处理组明显提高.对萘醌类代谢物谱的分析显示,SNP处理也引起新疆紫草愈伤组织中紫草素及其衍生物含量的增加.上述结果表明,SNP处理产生的NO,可能通过上调紫草素及其衍生物合成相关的基因表达,促进紫草素及其衍生物的合成.
Nitric oxide （NO） is an important signal molecule involved in the regulation of plant secondary metabolism. In this study, SNP （sodium nitroprusside） was utilized as the donor of nitric oxide to treat Arnebia euchroma callus. We find that the expression levels of the genes involved in the biosynthesis of shikonin and its derivatives, such as AePGT, AeGPPS, AeC4H, and Ae4CL, increase significantly in SNP-treated Red Strain callus tissues. Meanwhile, SNP treatment also increases the contents of some shikonin derivatives. The above-mentioned results indicate that exogenous NO increases the biosynthesis of shikonin and its derivatives by up-regulating the expression of the genes.
出处
《中国科学院大学学报（中英文）》
CAS
CSCD
北大核心
2014年第1期46-53,共8页
Journal of University of Chinese Academy of Sciences
基金
国家自然科学基金(61173098)
中国科学院"十二五"基础前沿专项(KSCX2-EW-J-29)资助
关键词
新疆紫草
一氧化氮
紫草素及其衍生物
硝普钠
基因表达
Arnebia euchroma
nitric oxide
shikonin and its derivatives
sodium nitroprusside
gene expression
