非病原细菌对烟草赤星病菌的生物抑制作用研究

Study of the biological control of non pathogenic bacteria to brown spot

从烟草根际土壤和烟草、黄瓜、番茄、桑树的茎、叶部位分离了 5 0株非病原芽孢杆菌和假单孢杆菌 ,在培养条件下对烟草赤星病菌进行了生物拮抗性测定。结果表明 ,非病原细菌对烟草赤星病菌有较强的拮抗作用。在不同来源的非病原细菌中 ,以来自烟草根际的非病原细菌对烟草赤星病菌的拮抗作用最强。在拮抗菌对目标菌烟草赤星病菌生长发育影响中发现 ,受拮抗菌作用 ,目标菌菌丝形态产生变异 ,生长发育受到影响。选取拮抗性较强的拮抗菌 ,在温室条件下处理 (喷洒 )烟苗 。

Fifty strains of non pathogenic bacteria were isolated from tobacco rhizospheric soil,stems and leaves of tobacco,cucumber,tomato and mulberry plants.The biological inhibitions of the strains were determinated under culture conditions.The results showed that the non plthogenic bacteria were inhibitive to Alternaria alternata.Among the fifty strains,the inhibition of the strains isolated from tobacco rhizospheric soil was the strongest.After treated by the antagonists,the formation appeared and growth of mycelium of Alternaria alternata was influnced.The brown spot was controlled efficiently after treated by the antagonists (BS5 and BS6)