铁炮百合快速繁殖技术研究

Study on the rapid propagation of Lilium formolongi

以铁炮百合(Lilium longiflorum)的鳞片、含苞待放时花蕾下的花梗为外植体,进行诱导生芽、生根的组织培养快速繁殖技术的研究。结果表明:鳞片诱导生芽最适合的初代培养基为MS+6-BA 2.0 mg/L+NAA 0.1 mg/L+2,4-D0.1mg/L;花梗的诱导生芽最适合的初代培养基为MS+6-BA 1.0 mg/L+NAA 0.5 mg/L;最佳继代培养基为MS+6-BA 3.0mg/L+NAA 0.02 mg/L;最佳生根培养基培养基为1/2MS+6-BA 0.1 mg/L+NAA 1.0 mg/L+活性炭0.1%;生芽率和生根率均可达100%。

Using the scales and peduncle as the explants, the tissue culture rapid propagation of Lilium formolongi was studied. The results showed that the primary culture optimal medium for inducing buds of scales was MS＋6-BA 1.0 mg/L＋NAA 0.5 mg/L＋2,4-D 0.1 mg/L; The primary culture optimal medium for inducing buds of peduncle was MS＋6-BA 2.0 mg/L＋NAA 0.1 mg/L;The secondary culture optimal medium was 6-BA 3.0 mg/L＋NAA 0.02 mg/L.The optimum rooting culture medium was 1/2MS＋6-BA 0.1 mg/L＋NAA 1.0 mg/L＋ activated carbon 0.1%; The budding rate and rooting rate was up to 100%o