4种多糖对山羊卵母细胞体外成熟的影响

Influence of four kinds of polysaccharide on goat's oocyte maturation in vitro

为优化卵母细胞体外成熟培养液,研究白术多糖、黄芪多糖、灵芝多糖及香菇多糖对卵母细胞体外成熟的作用。利用废弃卵巢收集未成熟卵母细胞,在成熟培养液中添加低(0.01、0.05 mg/mL)、中(0.10 mg/mL)、高(0.15、0.20 mg/mL)5个浓度梯度的4种多糖溶液培养卵母细胞。结果显示,4种多糖成熟培养液均在低浓度添加时对卵母细胞成熟率有明显的促进作用,以白术多糖效果最好,卵母细胞成熟率与对照组相比提高了27.28%,高浓度的多糖培养液对卵母细胞均有不同程度的抑制作用,导致细胞皱缩甚至死亡。低浓度的多糖培养液能够促进卵母细胞的成熟,并能提高卵母细胞进一步发育的能力。

In order to optimize the vitro maturation medium, effects of Soluble Polysaccharide, Astragalus Polysaccharide, Lucidum Polysaccharide and Lentinan Polysaceharide on oocyte maturation in vitro were studied. This test used the immature occytes from the abandoned ovarians, adding four kinds of polysaccharides with five different concentrations （0.01 mg/mL, 0.05 mg/mL, 0.10 mg/mL, 0.15 mg/mL, 0.20 mg/mL） in oocyte maturation medium. The result showed that four kinds of polysaccharide had promotion effect on oocyte mature in low concentration, especially the Soluble Polysaccharide, whose maturation rate raised 27.28% compared with the control group. The high concentration of polysaccharide had different degree inhibition to oocyte maturation, leading to cells shrivel and even death. Finally, the result indicated that the maturation medium with low concentration of polysaccharide would promote the oocyte maturation and further development.