樟脑型樟树芽器官离体培养技术

Culture Technology of Buds in Cinnamomum camphora with Camphor in Vitro

以樟脑含量＞70％的樟脑型樟树优株的嫩枝为外植体,进行芽器官离体培养技术研究.结果表明：每年的1～4月外植体灭菌消毒接种,无菌活体获取率比较高;适宜樟脑型樟树组培的基本培养基：M2 （MS＋Ca （NO3）2.4H2O 300 mg/L）;芽诱导培养激素组合：6-BA4 mg/L＋NAA1.5 mg/L＋KT 0.5 mg/L;芽增殖培养激素组合：6-BA3.0 mg/L ＋NAA0.5 mg/L ＋KT0.2 mg/L;在培养基中添加L-半光氨酸10 mg/L抑制芽褐化效果良好.

Using young shoots on the plus trees of Cinnamomum camphora with the content of camphor 〉70% as explants, the culture technology of buds in vitro was studied. The results revealed that the ob- taining rate of the sterilized buds was higher from January to April by sterilization inoculation of ex- plants. The basic medium suited for Cinnamomum camphora with camphor was M2 （ MS ＋ Ca （ NO3 ） 2. 4H20 300 mg/L）. The hormone combination of bud induction was 6 - BA4 mg/L ＋ NAA1.5 mg/L ＋ KT0. 5 mg/L. The hormone combination of proliferation culture was 6 - BA3.0 mg/L ＋ NAA0. 5 mg/L ＋ KT0. 2 mg/L. The medium with L- Cysteine 10 mg/L could suppress the browning phenomenon effectively.