柚木茎段组培快繁技术

Stem Segment Tissue Culture and Rapid Propagation of Tectona grandis

以柚木（Tectona grandis）3年生优良无性系植株茎段为外植体,研究柚木组织培养快速繁殖技术.结果显示,用0.1％的HgCl2浸泡外植体4 min,无菌水清洗2次后,再用0.1％的HgCl2浸泡4 min的消毒效果最好,无菌活外植体得率30％左右;适宜茎段离体培养的基本培养基为MS;最佳的增殖培养基为MS ＋6-BA 1.0mg/L,芽增殖系数6.1以上;单芽在1/2 MS＋ NAA 1.0 mg/L＋IBA 0.5 mg/L培养基上的生根率达到90％以上,每株能长3～4条根,平均根长1.5～2.5 cm,根系健壮.

Using stem segments of 3-year-old superior Tectona grandis clone as explants, the tissue cul- ture and rapid propagation were studied. The results showed that the yield of sterile survival explants reached to 30% , when the explants were soaked into 0. 1% HgC12 for 4 rain and washed by sterile water for 2 times before soaking into 0. 1% HgC12 for 4 min again. MS was the suitable basic culture medium for isolated culture of stem segments. The optimal propagation culture medium was MS ＋ 6-BA 1.0 mg/L. The bud multiplication coefficient could reach above 6. 1. The rooting rate of single bud reached to 90% with culture medium of 1/2MS ＋ NAA1.0 mg/L ＋ IBA 0. 5 mg/L, whicl could grow 3 - 4 strong roots with length ranging from 1.5 to 2. 5 em.