豚鼠过敏试验影响因素探讨

Study on the Factors about Hypersensitive Reaction of Guinea Pig

目的探讨对豚鼠全身主动过敏试验的影响因素。方法分别研究过敏试验中使用的动物性别、试验药物中添加过敏原、再次激发试验对过敏反应发生率的影响。结果豚鼠的性别与过敏反应发生率并无明显相关性;添加的过敏原-卵白蛋白对注射用炎琥宁和注射用阿奇霉素的过敏试验无影响,但用卵白蛋白液激发时过敏反应程度与阳性组比较有明显差别;对激发后3h未死亡的动物进行再激发,结果阳性组动物又出现了剧烈的过敏反应,而添加过敏原的药物组无明显反应。结论豚鼠的性别及药物中添加过敏原-卵白蛋白对药物过敏反应发生率无明显影响,再激发后过敏发生率与致敏情况有关。

Objective To discuss the factors on hypersensitive reaction of guinea pig using type I hypersensitive model. Methods To investigate the effects of the animals＇ sex,the allergen appended in the test drug, the repeated injection of albumin on the frequency of anaphylaxis, respectively. Results There was no correlation between the sex of guinea pig and the incidence of hypersensitive reaction. The allergen albumin appended in test of drug did not produce effects on hypersensitive reaction of the drug, there were different reactions with repeated allergen injection. Reactivating animals who were survival after activating for 3 h, and the positive group of animals had a severe allergic reaction, while the group appended allergen drug had no obvious reaction. Conclusion The hypersensitivity of guinea pig is not related to the gender and the addition of albumin to the test drug, but appears to the allergen sensitization.