复发性阿弗他溃疡Th1/Th2细胞因子的检测

Expression of Thl/Th2-Related Cytokines in Recurrent Aphthous Ulcers

目的通过检测复发性阿弗他溃疡(recurrent aphthous ulcer,RAU)患者病程中及正常人群唾液及血液中Th1/Th2相关细胞因子IL-2、INF-r及IL-6的含量及变化,观察Th1/Th2相关细胞因子含量变化与RAU病程之间的相关性。方法收取RAU患者20人,正常对照组10人,RAU患者于口腔溃疡病程2、6、10d取样,分别采集静脉血样及唾液样本,双抗夹心ELISA法检测IL-2、IFN-γ及IL-6含量。结果血液中IL-2的含量在RAU患者3次取样中均较正常人群降低,其结果差异有统计学意义(P<0.05),唾液中IL-2含量在RAU患者3次取样中均较正常人群降低,但其结果差异不具有统计学意义(P>0.05)。血液及唾液中IFN-γ含量在RAU患者3次取样中均较正常人群降低,但其结果差异不具有统计学意义(P>0.05)。血液及唾液中IL-6含量在RAU患者3次取样中均较正常人群升高,但其结果差异不具有统计学意义(P>0.05)。结论细胞因子IL-2、IFN-γ及IL-6在RAU患者中均出现不同程度的变化,RAU患者出现Th1/Th2型免疫紊乱、Th1型免疫抑制、Th2型免疫激活可能为病因之一。

Objective To investigate the change and clinical significance of Thl/Th2 related cytokines in saliva and peripheral blood of the patients of recurrent aphthous ulcer（RAU）. Methods 20 RAU patients and 10 healthy controls were enrolled in this study. The saliva and peripheral blood samples were collected on the 2,6 and 10 day after the ulcer occurred respectively. The levels of IL-2, IFN ~ and IL-6 in saliva and peripheral blood were detected by ELISA. Results The IL-2 levels were lower in the experimental group than in the control group. The concenlralion,s of II,-2 in serum were significantly lower in the test group than in the conlrol group, while the concentration of II. 2 in saliva showed no slalistically significant difference. The IFN ）＇ levels were also lower in the experimental grouI） than in die control group, But there were no statistically significant difference in both serum and saliva. The II.-6 level was higher in the experimental group than in the control group,lint there were no statistically significant difference both in serum and saliva. Conclusion There were certain kinds of change in the inflammatory cytokine levels in RAU patients,the immune dysregulation may be one of the key factors related to the development of RAU.